Microbial dynamics in rearing trials of Hermetia illucens larvae fed coffee silverskin and microalgae

In the present study, Hermetia illucens larvae were reared on a main rearing substrate composed of coffee roasting byproduct (coffee silverskin, Cs) enriched with microalgae (Schizochytrium limacinum or Isochrysis galbana) at various substitution levels. The microbial diversity substrates, larvae, and frass (excrement from mixed residue) studied by combination culturing growth media metataxonomic analysis (Illumina sequencing). High counts total mesophilic aerobes, bacterial spores, presumptive lactic acid bacteria, coagulase-positive cocci, eumycetes detected. Enterobacteriaceae low in diets, whereas higher this family observed frass. microbiota substrates was characterized presence including genera Lactobacillus, Leuconostoc Weissella. H. fed Cs dominance Paenibacillus. diets containing I. galbana Enterococcus, Lysinibacillus, Morganella, Paenibacillus, depending algae inclusion level, while S. high relative abundances Brevundimonas, Paracoccus, level. Brevundimonas Alcaligenes dominated galbana; predominance also Schyzochitrium-enriched diets. Based results an effect nutrient bioactive substances (e.g. polysaccharides, high-unsaturated fatty acids, taurine, carotenoids) abundance some taxa detected may be hypothesized, thus opening new intriguing perspectives for control entomopathogenic species foodborne human pathogens potentially occurring edible insects. Further studies are needed to support hypothesis. Finally, information insect obtained.